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The materials employed in the fabrication of organ-on-chip systems play a decisive role in the prediction performance of these systems 44 PDMS has been widely used for microfluidics-based organ-on-chip platforms and microphysiological-system fabrication 25,45,46,47 PDMS has several beneficial properties, such as optical transparency for cell imaging and assessment, low autofluorescence, good gas permeability, and biocompatibility for cell cultures in an enclosed microfluidic device 44 In addition, PDMS also offers low-cost, rapid and convenient fabrication and fast turnaround times to prototype systems

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